Patient 1 was a 55-year-old male with cerebral infarction due to obstruction of the left middle cerebral artery during treatment for bacteremia, along with a verruca of infectious endocarditis harvested from endovascular thrombectomy. Patient 2 was a 59-year-old female suffering from cerebral infarction at the terminal branch during intrahepatic cholangiocarcinoma chemotherapy who thereafter developed cerebral infarction again due to obstruction of the left middle cerebral artery, along with a verruca of nonbacterial thrombotic endocarditis (NBTE) harvested from endovascular thrombectomy. In tumor-bearing patients, while NBTE may be more closely related to the development of cerebral infarctions than previously assumed, we also need pay attention to the onset of infectious endocarditis. We need further studies on the effectiveness and safety of thrombolysis therapy and endovascular thrombectomy for cerebral infarctions due to endocarditis in both patients. The harvested emboli may provide clues to the differentiation thereof.An 89-year-old man was admitted because of persistent fever and impaired consciousness. On admission, his consciousness level was E3V3M4 according to the Glasgow Coma Scale. MRI of the brain showed high intensity lesions in the bilateral cingulate gyri. In the cerebrospinal fluid, both cell counts and glucose level were in the normal ranges. He had received antibiotics and intravenous isotonic saline. On the fifth day of hospitalization, blood examination revealed elevation of anti-herpes simplex virus (HSV) immunoglobulin M antibody, and herpes simplex encephalitis (HSE) was diagnosed. Despite treatment with acyclovir, his respiratory function and consciousness level deteriorated rapidly. On the eighth day, he died of respiratory failure. At autopsy, the brain showed multiple softenings of the gray and white matter in the hippocampus, amygdala, and temporal, insular, and cingulate cortices. Some of these lesions were hemorrhagic. Microscopic examination revealed that the lesions were necrotic and associated with perivascular inflammatory cell infiltration in the limbic system, hypothalamus, brainstem tegmentum area, and medulla. Eosinophilic intranuclear inclusions were rarely found in the astrocytes in the medulla. Immunohistochemistry revealed anti-HSV-1 antibody positive neurons in the brainstem tegmentum including reticular formation and the raphe nuclei. HSV-DNA was also detected in the postmortem cerebrospinal fluid. This was a rare case of HSE in which inflammation in the brainstem proved to be the cause of lethal respiratory failure.Immunochromatography assay is an easy and rapid on-site detection method. However, conventional sandwich immunochromatographies using two antibodies can only detect target molecules above a threshold size. Small molecules below 1000 in molecular weight are usually detected using competitive immunoassay. However, competitive immunoassay is not suitable for visual detection of low concentration samples. Based on the principles of open sandwich immunoassay, which detects small molecules via interchain interaction of separated variable region fragments (VH and VL) from a single antibody, we developed non-competitive open sandwich immunochromatography. Bone Gla protein (BGP)-C7, a peptide containing the seven C-terminal amino acids of human osteocalcin, was selected as the target. By using VL fragments fixed on a nitrocellulose membrane, and colored cellulose bead-labeled VH fragments, we specifically detected 10 ng/mL of BGP-C7. This is the first report of open sandwich immunochromatography, which is an easy and rapid method for on-site, signal-on detection of small molecules.We investigated a host-guest complex between cucurbit[7]uril and malachite green, and its effect on the toxicity to human liver cells. The host-guest complexation was evaluated by a UV/vis titration and electrospray-ionization mass spectrometry. Interestingly, the host-guest complex resulted in remarkable suppression of the toxicity of malachite green in its practical concentration range (ca. ∼6 μM). This study is one step forward to the active control of the biological effects of potent toxicants utilizing host-guest chemistry.High-value agricultural products are characterized by the geographical conditions of the production areas such as climatic and soil conditions. These products are protected by the geographical indication (GI) protection system, which has been introduced in more than 100 countries. Because GI products are expensive in the market, products are often mislabeled as GI. Thus, there is an urgent need for the development of analytical methods that enable the tracing of geographical origins of food materials. Stable isotope analysis is used to trace the geographical origin of food materials. In this study, we review the applications for tracing the geographical origin of agricultural products (especially rice, beef, and honey) focusing on an analytical method for analyzing stable isotopes (δD, δ13C, δ15N, δ18O, and δ34S).Thermal analysis and calorimetry share a close relationship in the field of thermal research. With regards to the specific heat capacity, researchers have been able to realize absolute measurement techniques by utilizing drop, conduction, and adiabatic methods that are used in calorimetry. Furthermore, it is possible to optimize differential scanning calorimetry, which is a comparative measurement technique for the specific heat capacity used in thermal analysis, by improving the absolute measurement techniques. At the National Metrology Institute of Japan (NMIJ), we developed a new certified reference material (CRM) for comparatively measuring the specific heat capacity, the single-crystalline silicon-NMIJ CRM 5806a, using a new type of cryogenic adiabatic calorimeter equipped with a pulse-tube refrigerator working in the temperature range from 50 to 350 K. This CRM was produced in accordance with the quality specifications of NMIJ, and complies with the ISO/IEC 17025, ISO 17034, and ISO GUIDE 35 standards. This paper reports on the procedure for fabricating this CRM and using it to perform specific heat capacity measurements at low temperatures. DMB The specific heat capacity was measured using a differential scanning calorimeter in the temperature range from 280 to 340 K. NMIJ CRM 5806a was used to calibrate the heat flow. It was found that the uncertainty evaluation became easier because one factor of the uncertainty evaluation could be removed using the CRM. We show that the development of the CRM using the adiabatic calorimeter has led to an improvement in the specific heat capacity measurement results obtained by the differential scanning calorimeter.