Poor knee biomechanics in a volleyball spike jump generally result in a higher knee injury risk, which can be altered by an internal focus of attention (FOA). The constrained action hypothesis (CAH) purports that the FOA inhibits sports performance whereas no ecologically valid evidence has been found in previous studies. The purpose of this research is to explore the effect of video feedback on knee biomechanics in a volleyball spike jump including landing and take-off phases. The video feedback was performed in a natural way. Fourteen volleyball male players were recruited in this study. A paired t-test was used to detect the effect of the feedback; meanwhile, statistical parameter mapping (SPM) statistics were used for the continuum differences during movement. After biofeedback, the initial contact flexion angle of the knee (t = 2.179, p = 0.049), the maximal flexion angle of the knee (t = 3.242, p = 0.006) and the maximal internal rotation angular velocity of the knee (t = 5.209, p = 0.003) increased sig) and an external moment of the knee during the phase from 85.55 to 95.06% (t = 4.214, p less then 0.001); the vGRF significantly decreased during the phase from 3.13 to 5.94% (t = 4.096, p = 0.014) and 19.83-21.97% (t = 4.096, p = 0.024) but significantly increased in the phase of 91.43-100% (t = 4.096, p less then 0.001). The impulse of the vGRF and knee power were not found to be different compared with before biofeedback. Therefore, our study suggests video feedback in a natural practice has the potential to improve knee movement whilst not altering the performance in a volleyball spike jump. This indicates that the CAH theory is possibly not suitable in a real competition. Due to the complexity of human movements and the limitations of this study, muscle activities must be considered in the future.Lingula anatina is a brachiopod widely distributed in the western Pacific region. Even though L. anatina has been targeted for a number of biological studies, there is still limited information on intraspecific genetic variations of L. anatina. In this study, L. anatina specimens were collected from Korea and Vietnam, and complete mitochondrial genome (mitogenome) sequences were analyzed and compared with previous records. The total mitogenomes of L. anatina were 24,875 bp and 25,305 bp in size for Korean and Vietnamese specimens, respectively. Those mitogenomes are extraordinarily longer than the typical mitogenome size for an animal but shorter than the previous record from Yanagawa (Japan) for this species. The gene orders and the sizes of the protein-coding genes are also different from those for the Japanese specimen. Furthermore, the nonsynonymous (Ka) and synonymous (Ks) substitution rates in protein-coding genes (PCGs) were calculated to test the idea of evolutionary rate differences in mitochondrial genomes. VS-4718 mouse The analyses showed relatively low Ka and Ks for the complete mitogenomes from Buan (Korea), Doson (Vietnam) and Yanagawa (Japan). The Ka/Ks ratio was less than 1 in comparisons of three localities, indicating the existence of purifying selection in this species. The phylogenetic analyses showed that L. anatina diverged among localities in the western Pacific region.Particle mixing process is critical for the design and quality control of concrete and composite production. This paper develops an algorithm to simulate the high-shear mixing process of a granular flow containing a high proportion of solid particles mixed in a liquid. DEM is employed to simulate solid particle interactions; whereas SPH is implemented to simulate the liquid particles. The two-way coupling force between SPH and DEM particles is used to evaluate the solid-liquid interaction of a multi-phase flow. Using Darcy's Law, this paper evaluates the coupling force as a function of local mixture porosity. After the model is verified by two benchmark case studies, i.e., a solid particle moving in a liquid and fluid flowing through a porous medium, this method is applied to a high shear mixing problem of two types of solid particles mixed in a viscous liquid by a four-bladed mixer. A homogeneity metric is introduced to characterize the mixing quality of the particulate mixture. The virtual experiments with the present algorithm show that adding more liquid or increasing liquid viscosity slows down the mixing process for a high solid load mix. Although the solid particles can be mixed well eventually, the liquid distribution is not homogeneous, especially when the viscosity of liquid is low. The present SPH-DEM model is versatile and suitable for virtual experiments of particle mixing process with different blades, solid particle densities and sizes, and liquid binders, and thus can expedite the design and development of concrete materials and particulate composites.Evidence about the impact of vegetable and fruit diversity consumption on the autonomic nervous system (ANS) functioning is scarce. In this cross-sectional study (513 participants, 49.9% girls aged 7 to 12 years), we evaluated the association between vegetable and fruit diversity consumption and the ANS in school-aged children. Dietary intake was collected using a single 24-h recall questionnaire. Fruit and vegetable diversity consumption was estimated by summing up all the different individual vegetables and fruits consumed in one day. Pupillometry was used to assess pupillary light response, which evaluated the ANS activity. Adjusted linear regressions estimated the association between vegetable and fruit diversity consumption with pupillary light response measures. There was a positive and significant association between vegetable diversity consumption and the average dilation velocity, a measure related to the sympathetic nervous system activity (β-coefficient = 0.03, 95%CI 0.002; 0.07). Our findings show that vegetable diversity consumption is associated with the ANS response, a possible early link between diet and health in school-aged children.Different factors may contribute to the development of osteopenia or osteoporosis. Fatty acids are key nutrients for health, and a number of studies have reported an association between bone mineral density (BMD) and fatty acid intake. We aimed to investigate the relationships between serum levels of different fatty acids and bone parameters determined by quantitative bone ultrasound (QUS), peripheral quantitative computed tomography (pQCT), and dual-energy X-ray absorptiometry (DXA) in a sample of Spanish postmenopausal women. We enrolled a total of 301 postmenopausal women (median age 59 years; interquartile range (IQR) 7) in this study. All participants underwent full densitometric screening, including calcaneal quantitative ultrasound (QUS), peripheral quantitative computed tomography (pQCT), and dual-energy X-ray absorptiometry (DXA), as well as plasma fatty acid measurement. After adjustment for potential confounders, plasma n-3 polyunsaturated fatty acid (PUFA) levels correlated with BMD in the spine (r = 0.