Comparison of an effect of imidazolium and sodium salts on acidic and alkaline transitions and to thermal transition of cyt c implies a role of hydrophobic interaction between imidazolium cation and polypeptide chain.Herein, thermo- and pH-sensitive pectin-graft-poly(dimethylaminoethyl methacrylate) copolymer-coated magnetic nanoparticles were synthesized via a green and rapid synthetic approach based on microwave irradiation. Firstly, a novel thermo- and pH-sensitive pectin-graft-poly(dimethylaminoethyl methacrylate) copolymer (Pec-g-PolyDMAEMA) was synthesized and then, Pec-g-PolyDMAEMA based magnetic nanoparticles (Pec-g-PolyDMAEMA@Fe3O4) were produced via microwave-assisted co-precipitation method. The thermo/pH/magnetic field multi-sensitive hybrid nanoparticle was characterized by techniques like TEM, VSM, FT-IR, and TGA/DSC. In vitro release studies of 5-Fluorouracil (FL) were carried out by altering the temperature (37 and 44°C), pH (5.5 and 7.4) and presence of an AMF. The FL release of Pec-g-PolyDMAEMA@Fe3O4@FL exhibited pH-sensitive behavior. They showed thermo/pH-sensitive FL release features with the greatest release of FL at 37°C (56%) than at 44°C (40%) and at pH of 7.4 (63%) than at pH of 5.5 (45%) within 48h. The FL release was also significantly increased (100%) with the presence of a 50 mT magnetic field. These results indicate that the developed Pec-g-PolyDMAEMA@Fe3O4 nanoparticles are promising in the application of multi-stimuli-sensitive delivery of drugs.Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions. This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film. The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77. The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated. SEM images showed that the CNC was evenly distributed in the CMC matrix to form homogenous films when the content of CNC was ≤5 wt%. The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability. selleck compound Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film. Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies. These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.The push-pull solvatochromic pyrene derivatives PA and PK have been applied to the study of model membrane vesicles, cells and purified human serum lipoproteins, using both confocal fluorescence microscopy and fluorescence spectroscopy. These polarity-sensitive probes provide information similar to that obtained by Laurdan or Prodan, i.e. mainly lipid order in biomembranes, but they have the essential advantage of being excitable by a standard 405 nm laser light, bypassing the use of multiphoton excitation. In addition, they are brighter and much more photostable than those dimethylamino naphthalene derivatives. Our results with model membrane spectroscopy (multilamellar vesicles) and with microscopy (giant unilamellar vesicles) showed the capacity of PA and PK to report differently on liquid-disordered, liquid-ordered and gel phase bilayers. Moreover, a ratiometric parameter, the Red/Blue Intensity Ratio (RBIR) could be used for inter-domain, inter-vesicle and even inter-technique comparison, and the appropriate microscopy-spectroscopy conversion coefficients could be estimated. In studies at the cellular level, PA probe stained almost exclusively the plasma membrane of red blood cells, revealing its high degree of lipid order. Using Chinese Hamster Ovary cells PA was shown to be an excellent probe for the detection of cytoplasmic lipid droplets, superior to Nile Red in that PA provides simultaneously a detailed information of membrane order in the whole cell, in which the lipid droplets appear with a very good contrast. Moreover, spectrofluorometric data of PA-stained serum lipoproteins indicated an essentially identical value of RBIR for lipid droplets and for high-density lipoproteins.As a common negative emotion in daily life, regret is harmful to mental health and even inclined to induce mental illness. Therefore, to modulate and reduce regret is of wide concern. The current fMRI study aimed to investigate the modulation of attentional deployment training on subsequent regret by using a sequential risk taking task. On each trial of the task, participants were asked to open a series of boxes consecutively and decided when to stop. Each box contained a reward, except for one containing a devil to zero one's gain in the trial. After participants stopped, both collected gains and missed chances were revealed. The study consisted of training and testing stages. At training stage, attentional deployment manipulation was carried out during the outcome feedback of the task, i.e. participants were induced to focus on gains or missed chances by highlighting different parts of the outcome. In the testing stage followed, participants completed the task with no attentional deployment manipulation. Behaviorally, after a training stage focusing on gains, participants felt less regret when encountered with worse outcome at testing stage. At the neural level, more activities of reward related brain regions such as right putamen and cognitive control related brain regions such as dmPFC, SFG and MFG were observed at testing stage followed by a training stage focusing on gains. The current study highlighted that attentional deployment training could modulate the subsequent regret effectively, and that dmPFC, SFG and MFG played a key role in this process.