The objective of this study was to identify the calf-level colostrum management practices associated with an adequate transfer of passive immunity (TPI; defined as serum Brix refractance ≥8.4% in the first week of life) in small-sized herds. A total of 818 calves from 61 commercial Holstein dairy farms were included in this observational cross-sectional study. For each calf, sex, colostrum delivery method, colostrum volume fed at first meal, and time to first feeding (delay between birth and first colostrum meal) were noted. Blood and colostrum samples were collected to estimate the serum and colostrum quality using Brix refractometry. To quantify the level of bacterial contamination in colostrum samples, total bacteria count and total coliform count (TCC) were measured using the Petrifilm (3M, St. Paul, MN) culture system. In this study, 68% of calves had an adequate TPI (≥8.4%). For data distribution, the 25th, 50th, and 75th percentiles were 1.3, 2.8, and 3.3 L for the colostrum volume fed at the first meal; 20.9, 23.5, and 26.5% Brix; and 1.1, 3.1, and 6.5 h for the time to first feeding of colostrum, respectively. The odds of adequate TPI were 2.6 times higher in calves receiving ≥2.5 L colostrum at their first meal, 2.9 times higher in calves receiving colostrum with ≥24.5% Brix, and 1.6 times higher in calves receiving colostrum within 3 h after birth, than in calves not meeting these criteria. In the present study, median bacterial contamination distribution (interquartile range) in the first colostrum meal was 14,000 cfu/mL (3,000-83,000 cfu/mL) for total bacteria count, and 0 cfu/mL (0-1,000 cfu/mL) for TCC. Total bacteria count and TCC were not associated with the odds of adequate TPI in the final model. Overall, these results suggest that specific calf-level colostrum management practices are associated with adequate TPI in small- to medium-sized dairy herds.Increased concentrations of some serum biomarkers are known to be associated with impaired health of dairy cows. Therefore, being able to predict these biomarkers, especially in the early stage of lactation, would enable preventive management decision. Some health biomarkers may also be used as phenotypes for genetic improvement for improved animal health. In this study, we validated the accuracy and robustness of models for predicting serum concentrations of β-hydroxybutyrate (BHB), fatty acids, and urea nitrogen, using milk mid-infrared (MIR) spectroscopy. The data included 3,262 blood samples of 3,027 lactating Holstein-Friesian cows from 19 dairy herds in Southeastern Australia, collected in the period from July 2017 to April 2020. The models were developed using partial least squares regression and were validated using 10-fold random cross-validation, herd-year by herd-year external validation, and year by year validation. The coefficients of determination (R2) for prediction of serum BHB, fatty acids, avel phenotypes for genetic improvement purposes.Skin bag Tulum cheeses traditionally produced in the Central Taurus region of Turkey were studied to identify peptide profiles by liquid chromatography-tandem mass spectrometry over 180 d of ripening. After mass spectrometry analysis, 203 peptides were identified 59 from αS1-casein (CN), 11 from αS2-CN, 129 from β-CN, and 4 from κ-CN. Numbers of αS1- and β-CN-derived peptides increased with increasing number of ripening days due to the dependence of newly formed peptides on proteolysis. However, similar increases were not observed for αS2- and κ-CN-derived peptides. Most identified peptides consisted of β-CN-derived peptides, followed by αS1-, αS2-, and κ-CN-derived peptides. Among these, bioactive peptides were found, including antihypertensive, antibacterial, antioxidant, dipeptidyl peptidase-4 inhibitory, metal chelating, skin regenerating, glucagon-like peptide-1 secretion enhancing, opioid, cathepsin B inhibitory, prolyl endopeptidase inhibitory, immunomodulatory, brain function improving, antiamnesic, antihypercholesterolemic, anti-inflammatory, and anticarcinogenic peptides.Despite high numbers of cattle, milk production in many tropical countries such as Ethiopia is very low. Animals are managed traditionally, meaning they mostly depend on seasonal availability of natural pasture, grass, and crop residues with no supplementary feeds. Due to the lack of pasture management, there is overgrazing and soil erosion, and the land still must deal with extremely dry periods. All this has a negative effect on dairy cow productivity. Identification of the specific nutritional deficits would enable targeted interventions to improve milk yield performance, but nutrient and energy intakes are difficult to assess in ranging conditions. The aim of this research was, therefore, to evaluate the nutritional status of ranging dairy cows through blood metabolites, milk yield, and body condition in relation to environmental factors such as agro-ecology and season. The study was performed in a tropical region that is known to be exposed to the above-mentioned situation, the Arba Minch region in the southern Ethiopian Rift Valley. Blood samples were collected from 170 ranging dairy cows in 6 different districts, along a transect extending from the lowlands to the highlands, in both seasons (dry and rainy). Body condition score and milk yield of all cows were also determined for both seasons. Serum urea, creatinine, triglyceride, and nonesterified fatty acid concentrations were quantified spectrophotometrically. Dried serum spots were subject to quantitative electrospray tandem mass spectrometry to estimate changes in nutrient metabolism based on selected free AA and carnitine esters. Based on these measurements, nutritional status varied with season and geographical region. It can also be concluded that extensive metabolite analysis such as mass spectrometry can provide detailed insights, but the simpler spectrophotometric metabolite analysis can estimate the nutritional status of ranging animals.We compared the management and housing of dairy heifers from calf to calving in herds that were very successful versus less successful in preventing mastitis in early lactation primiparous cows. This retrospective observational study included 170 Swedish dairy herds. Eligible herds were identified from the Swedish official milk recording scheme (SOMRS). Each herd had at least 60 cows per year, production data from 3 consecutive years, and at least 10 primiparous cows per year with their first milk recording 5 to 35 d after calving and their second milk recording 20 to 40 d after the first milk recording. In each herd, primiparous cows with a low (≤75,000 cells/mL) cow somatic cell count (CSCC) at both the first and second milk recording were categorized as low-low (LL); those with a high (>100,000 cells/mL) CSCC at both recordings were categorized as high-high (HH). read more Cows with high CSCC at the first recording and low at the second were categorized as high-low (HL). The annual proportions of LL, HL, and HH cows within each herd were calculated.